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精子膜评价的荧光技术
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JoVE Journal 発生生物学
Fluorimetric Techniques for the Assessment of Sperm Membranes
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精子膜评价的荧光技术

12,853 Views

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08:58 min

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November 28, 2018

DOI:

10.3791/58622-v

DOI:
10.3791/58622-v

•

08:58 min
November 28, 2018

•

27 Views
,
1Animal Sperm Research Center, Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem, 2Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment,Hebrew University of Jerusalem

概要

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在这里, 我们提出的方法来评估精子细胞膜的完整性, 一个细胞特征与精子受精能力。我们描述了精子膜荧光测量评估的三种技术: 与特定荧光探针同时染色, 荧光显微镜, 和先进的精子专用流式细胞仪。还介绍了合并这些方法的实例。

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Cite this Article

Komsky-Elbaz, A., Roth, Z. Fluorimetric Techniques for the Assessment of Sperm Membranes. J. Vis. Exp. (141), e58622, doi:10.3791/58622 (2018).

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