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Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

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09:16 min

November 26, 2018

DOI:

09:16 min
November 26, 2018

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概要

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Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.

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