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Immunology and Infection

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JoVE Journal Immunology and Infection
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
 

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Article DOI: 10.3791/58509-v • 09:16 min • November 26th, 2018
November 26th, 2018 •
1, 2, 1, 3, 3
1Hematology service, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty, University of Geneva

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Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.

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Keywords: Monocyte Subpopulations Leukocyte Adhesion Trans-endothelial Migration Hematopoietic Cells HUVEC CMFDA Density Gradient Separation PAN Monocyte Isolation Transmigration Adhesion M199 Medium BSA
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