/
/
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
6,663 Views
•
09:16 min
•
November 26, 2018
DOI:
1Hematology service, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva, 2Department of Pathology and Immunology, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva, 3Department of Cell Physiology and Metabolism, Centre Médical Universitaire (CMU), Medical faculty,University of Geneva
Summary
Automatically generated
Here, we present an integrated protocol that measures monocyte subpopulation trafficking under flow in vitro by use of specific surface markers and confocal fluorescence microscopy. This protocol can be used to explore sequential recruitment steps as well as to profile other leukocyte subtypes using other specific surface markers.
Read Article
Cite this Article
Ropraz, P., Imhof, B. A., Matthes, T., Wehrle-Haller, B., Sidibé, A. Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro. J. Vis. Exp. (141), e58509, doi:10.3791/58509 (2018).
Copy