We present a method to detect 3-nitrotyrosine chemical modifications of atmospheric proteins with 6 mm-diameter rounds cut from air sampler prefilters using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD).
Ito, T., Ogino, K., Nagaoka, K., Takemoto, K., Nishiyama, R., Shimizu, Y. Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System. J. Vis. Exp. (143), e58371, doi:10.3791/58371 (2019).