Detection of 3-Nitrotyrosine in Atmospheric Environments <em>via</em> a High-performance Liquid Chromatography-electrochemical Detector System

Tatsuo Ito; Keiki Ogino; Kenjiro Nagaoka; Kei Takemoto; Rina Nishiyama; Yurika Shimizu

doi:10.3791/58371

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Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

Journal JoVE
Environnement

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Journal JoVE Environnement

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

7,091 Views

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07:32 min

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January 30, 2019

DOI:

10.3791/58371-v

DOI:

10.3791/58371-v

•

07:32 min

January 30, 2019

•

5 Views

Tatsuo Ito¹, Keiki Ogino¹, Kenjiro Nagaoka¹, Kei Takemoto¹, Rina Nishiyama¹, Yurika Shimizu²

¹Department of Public Health,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, ²Department of Pathophysiology-Periodontal Science,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

Summary

We present a method to detect 3-nitrotyrosine chemical modifications of atmospheric proteins with 6 mm-diameter rounds cut from air sampler prefilters using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD).

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Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

Summary

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