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Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
JoVE 杂志
环境科学
This content is Free Access.
JoVE 杂志 环境科学
Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

DOI:
10.3791/58371-v

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07:32 min

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January 30, 2019

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, , , , ,
1Department of Public Health,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2Department of Pathophysiology-Periodontal Science,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

Chapters

  • 00:04Title
  • 00:28Collection and Hydrolysis of Proteins in Particulate Matter (PM)
  • 03:59Determination of 3-Nitrotyrosine (3-NT) Content via a High-Performance Liquid Chromatography and Electrochemical Detection (HPLC-ECD) System
  • 06:06Results: Detection of 3-NT in PM2.5, PM7, and Total Suspended Particle (TSP) Samples Using HPLC-ECD
  • 07:19Conclusion

Summary

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We present a method to detect 3-nitrotyrosine chemical modifications of atmospheric proteins with 6 mm-diameter rounds cut from air sampler prefilters using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD).

Tags

3-NitrotyrosineHPLC-electrochemical DetectionAir SamplingParticulate MatterEnvironmental ResearchOxidative DamageAllergies

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