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Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
JoVE 杂志
环境科学
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JoVE 杂志 环境科学
Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System

7,091 Views

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07:32 min

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January 30, 2019

DOI:

10.3791/58371-v

DOI:
10.3791/58371-v

•

07:32 min
January 30, 2019

•

5 Views
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1Department of Public Health,Okayama University Graduate School of Medicine, Dentistry, and Pharmaceutical Sciences, 2Department of Pathophysiology-Periodontal Science,Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences

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We present a method to detect 3-nitrotyrosine chemical modifications of atmospheric proteins with 6 mm-diameter rounds cut from air sampler prefilters using a high-performance liquid chromatography-electrochemical detector (HPLC-ECD).

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Cite this Article

Ito, T., Ogino, K., Nagaoka, K., Takemoto, K., Nishiyama, R., Shimizu, Y. Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System. J. Vis. Exp. (143), e58371, doi:10.3791/58371 (2019).

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