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High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
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10:12 min
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June 29, 2018
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概要
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Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.
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Kosuta, C., Daniel, K., Johnstone, D. L., Mongeon, K., Ban, K., LeBlanc, S., MacLeod, S., Et-Tahiry, K., Ekker, M., MacKenzie, A., Pena, I. High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping. J. Vis. Exp. (136), e58024, doi:10.3791/58024 (2018).
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