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High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
Capítulos
- 00:04Título
- 00:48Fin Clipping of Zebrafish Larvae
- 04:03Genomic DNA Extraction
- 04:53Alternative Genomic Extraction Using a Chelating Resin
- 05:40Multiplex PCR Assay
- 06:37Heteroduplex Melting Assay
- 07:39Results: Efficient Genotyping of Zebrafish Larvae by Fin Clipping
- 09:30Conclusion
Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.
