Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping
13,516 Views
•
10:12 min
•
June 29, 2018
DOI:
Summary
Automatically generated
Zebrafish have been used as reliable genetic model organisms in biomedical research, especially with the advent of gene-editing technologies. When larval phenotypes are expected, DNA extraction and genotype identification can be challenging. Here, we describe an efficient genotyping procedure for zebrafish larvae, by tail clipping, as early as 72-h post-fertilization.
Read Article
Cite this Article
Kosuta, C., Daniel, K., Johnstone, D. L., Mongeon, K., Ban, K., LeBlanc, S., MacLeod, S., Et-Tahiry, K., Ekker, M., MacKenzie, A., Pena, I. High-throughput DNA Extraction and Genotyping of 3dpf Zebrafish Larvae by Fin Clipping. J. Vis. Exp. (136), e58024, doi:10.3791/58024 (2018).
Copy