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Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
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JoVE Journal 遺伝学
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

DOI:
10.3791/57802-v

•

09:14 min

•

June 28, 2018

•

, , ,
1Molecular Physiology and Biophysics,University of Iowa

章

  • 00:04標題
  • 00:50Mapping Fastq Files
  • 02:14Bioinformatics Processing Using DEEPN Software
  • 06:08Verification of Candidates Identified by DEEPN
  • 08:01Conclusion

概要

自動翻訳

自動翻訳

Deep sequencing of yeast populations selected for positive yeast 2-hybrid interactions potentially yields a wealth of information about interacting partner proteins. Here, we describe the operation of specific bioinformatics tools and customized updated software to analyze sequence data from such screens.

タグ

Informatic AnalysisSequence DataBatch Yeast 2-hybrid ScreensProtein InteractomesBioinformatics ProcessingUser-friendly Graphic User InterfaceMolecular BiologistsCell BiologistsBiochemistsMAPster SoftwareDEEPN AnalysisHISAT2Multi-threadingReference DNA GenomeY2H Prey Library InsertsMapped FilesUnmapped Reads

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