Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
Capítulos
- 00:04Título
- 00:50Mapping Fastq Files
- 02:14Bioinformatics Processing Using DEEPN Software
- 06:08Verification of Candidates Identified by DEEPN
- 08:01Conclusion
Deep sequencing of yeast populations selected for positive yeast 2-hybrid interactions potentially yields a wealth of information about interacting partner proteins. Here, we describe the operation of specific bioinformatics tools and customized updated software to analyze sequence data from such screens.
Tags
Informatic AnalysisSequence DataBatch Yeast 2-hybrid ScreensProtein InteractomesBioinformatics ProcessingUser-friendly Graphic User InterfaceMolecular BiologistsCell BiologistsBiochemistsMAPster SoftwareDEEPN AnalysisHISAT2Multi-threadingReference DNA GenomeY2H Prey Library InsertsMapped FilesUnmapped Reads
