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Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
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June 28, 2018
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Deep sequencing of yeast populations selected for positive yeast 2-hybrid interactions potentially yields a wealth of information about interacting partner proteins. Here, we describe the operation of specific bioinformatics tools and customized updated software to analyze sequence data from such screens.
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Informatic AnalysisSequence DataBatch Yeast 2-hybrid ScreensProtein InteractomesBioinformatics ProcessingUser-friendly Graphic User InterfaceMolecular BiologistsCell BiologistsBiochemistsMAPster SoftwareDEEPN AnalysisHISAT2Multi-threadingReference DNA GenomeY2H Prey Library InsertsMapped FilesUnmapped Reads
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Krishnamani, V., Peterson, T. A., Piper, R. C., Stamnes, M. A. Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens. J. Vis. Exp. (136), e57802, doi:10.3791/57802 (2018).
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