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Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
1Systems Neurophysiology Research Group, Institute of Clinical Neuroscience and Medical Psychology, Medical Faculty,Heinrich Heine University Düsseldorf, 2Behavioural Neurodynamics Group,Leibniz Institute for Molecular Pharmacology (FMP)/ NeuroCure Cluster of Excellence, 3Neuronal Circuits and Behavior Research Group,Max Planck Institute for Metabolism Research
章
- 00:04標題
- 00:39Viral Injection
- 01:52Preparation of Optic Fibers
- 02:45Preparation of Tungsten Wire Arrays for LFP Recordings
- 03:56Stereotaxic Implantation
- 05:26Optogenetic Stimulation and Electrophysiological Data Acquisition
- 06:15Results: Optogenetic Entrainment of Theta Rhythm
- 07:02Conclusion
We describe the use of optogenetics and electrophysiological recordings for selective manipulations of hippocampal theta oscillations (5-10 Hz) in behaving mice. The efficacy of the rhythm entrainment is monitored using local field potential. A combination of opto- and pharmacogenetic inhibition addresses the efferent readout of hippocampal synchronization.
