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Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
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JoVE Journal 生化学
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

13,550 Views

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08:29 min

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April 27, 2018

DOI:

10.3791/57225-v

DOI:
10.3791/57225-v

•

08:29 min
April 27, 2018

•

12 Views
, , ,
1小児科,Emory University School of Medicine, 2Emory College of Arts and Sciences,Emory University

概要

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Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

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Cite this Article

Maxwell, J. T., Tsai, C., Mohiuddin, T. A., Kwong, J. Q. Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells. J. Vis. Exp. (134), e57225, doi:10.3791/57225 (2018).

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