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Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

13,550 Views

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08:29 min

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April 27, 2018

DOI:

10.3791/57225-v

DOI:
10.3791/57225-v

•

08:29 min
April 27, 2018

•

12 Views
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1Department of Pediatrics,Emory University School of Medicine, 2Emory College of Arts and Sciences,Emory University

Summary

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Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

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Maxwell, J. T., Tsai, C., Mohiuddin, T. A., Kwong, J. Q. Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells. J. Vis. Exp. (134), e57225, doi:10.3791/57225 (2018).

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