Journal
/
Biochemie
/
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

DOI:
10.3791/57225-v

•

08:29 min

•

April 27, 2018

•

, , ,
1Department of Pediatrics,Emory University School of Medicine, 2Emory College of Arts and Sciences,Emory University

Kapitel

  • 00:04Titel
  • 00:56Mitochondrial Calcium Uptake Measurement in Isolated Cardiac Mitochondria Using a Plate Reader
  • 03:33Mitochondrial Calcium Measurement Using Confocal Imaging in Cultured Cells
  • 06:14Results: Mitochondrial Calcium Analysis in Isolated Mitochondria and in Cultured Cells
  • 07:37Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

Tags

Mitochondrial Calcium InfluxCalcium SignalingMitochondrial Calcium DynamicsDisease ConditionsPlate-reader-based Mitochondrial Calcium UptakeConfocal-microscope-based Mitochondrial Calcium InfluxIsolated HeartMSEGTA BufferCalcium Green-5NCalcium Chloride

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Using Scaffold Liposomes to Reconstitute Lipid-proximal Protein-protein Interactions In Vitro

Nitrogen Cavitation and Differential Centrifugation Allows for Monitoring the Distribution of Peripheral Membrane Proteins in Cultured Cells

Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana

Interactions with and Membrane Permeabilization of Brain Mitochondria by Amyloid Fibrils

Induction of Eryptosis in Red Blood Cells Using a Calcium Ionophore

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Author Spotlight: Oxygen-Independent Assays to Measure Mitochondrial Function in Mammals

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Author Spotlight: Unveiling Mitochondrial Contact Sites and Architectural Insights

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Read Article