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Misura in tempo reale di permeabilità della barriera epiteliale in Organoids intestinale umana
JoVE Journal
発生生物学
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JoVE Journal 発生生物学
Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids
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Misura in tempo reale di permeabilità della barriera epiteliale in Organoids intestinale umana

13,765 Views

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08:04 min

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December 18, 2017

DOI:

10.3791/56960-v

DOI:
10.3791/56960-v

•

08:04 min
December 18, 2017

•

4 Views
, , , ,
1Department of Internal Medicine, Division of Gastroenterology,University of Michigan, 2Department of Cell and Developmental Biology,University of Michigan, 3Department of Internal Medicine, Division of Infectious Disease,University of Michigan

概要

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Questo protocollo descrive la misura della permeabilità della barriera epiteliale in tempo reale dopo il trattamento farmacologico in organoids intestinale umana utilizzando la microscopia a fluorescenza e microscopia cellulare di vivere.

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Cite this Article

Hill, D. R., Huang, S., Tsai, Y., Spence, J. R., Young, V. B. Real-time Measurement of Epithelial Barrier Permeability in Human Intestinal Organoids. J. Vis. Exp. (130), e56960, doi:10.3791/56960 (2017).

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