Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Lauren A. Laboissonniere; Takuma Sonoda; Seul Ki Lee; Jeffrey M. Trimarchi; Tiffany M. Schmidt

doi:10.3791/55229

ジャーナル

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神経科学

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Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

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神経科学

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JoVE Journal 神経科学

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

13,538 Views

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11:26 min

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May 22, 2017

DOI:

10.3791/55229-v

DOI:

10.3791/55229-v

•

11:26 min

May 22, 2017

•

1 Views

Lauren A. Laboissonniere¹, Takuma Sonoda², Seul Ki Lee², Jeffrey M. Trimarchi¹, Tiffany M. Schmidt²

¹Department of Genetics, Development, and Cell Biology,Iowa State University, ²Department of Neurobiology,Northwestern University

概要

Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.

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Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

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