Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Single-cell RNA-Seq of Defined Subsets of Retinal …
JoVE Journal
Neuroscience

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Neuroscience
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
 

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Article DOI: 10.3791/55229-v • 11:26 min • May 22nd, 2017
May 22nd, 2017 •
1, 2, 2, 1, 2
1Department of Genetics, Development, and Cell Biology, Iowa State University, 2Department of Neurobiology, Northwestern University

Capítulos

Resumen
Automatic Translation

Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.

Tags

Keywords: Single-cell RNA-Seq Retinal Ganglion Cells Fluorescent Labeling Neuronal Subtype Cellular Diversity RNA Isolation Retinal Tissue Dissociation Patch-clamp Electrophysiological Recordings Micropipette Puller Extracellular Solution Oxygenation Tissue Mounting Fluorescent Microscopy
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter