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Neuroscience

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JoVE Journal Neuroscience
Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
 

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Article DOI: 10.3791/55229-v • 11:26 min • May 22nd, 2017
May 22nd, 2017 •
1, 2, 2, 1, 2
1Department of Genetics, Development, and Cell Biology, Iowa State University, 2Department of Neurobiology, Northwestern University

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résumé
Automatic Translation

Here, we present a combinatorial approach for classifying neuronal cell types prior to isolation and for the subsequent characterization of single-cell transcriptomes. This protocol optimizes the preparation of samples for successful RNA Sequencing (RNA-Seq) and describes a methodology designed specifically for the enhanced understanding of cellular diversity.

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Keywords: Single-cell RNA-Seq Retinal Ganglion Cells Fluorescent Labeling Neuronal Subtype Cellular Diversity RNA Isolation Retinal Tissue Dissociation Patch-clamp Electrophysiological Recordings Micropipette Puller Extracellular Solution Oxygenation Tissue Mounting Fluorescent Microscopy
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