ジャーナル
/
発生生物学
/
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity
JoVE Journal
発生生物学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 発生生物学
Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

DOI:
10.3791/54934-v

•

08:30 min

•

February 24, 2017

•

, ,
1CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Division of Rheumatology, Seoul St. Mary’s Hospital, College of Medicine,The Catholic University of Korea

章

  • 00:05標題
  • 00:54Centrifugal Gravity Loading
  • 02:22Pellet Culture
  • 03:12Micromass Culture
  • 04:10Paraffin-embedding Cell Pellets
  • 05:59Results: Upregulation of Chondrogenic Differentiation Markers in Adipose-derived Stem Cells Following Centrifugal Gravity Loading
  • 07:59Conclusion

概要

自動翻訳

自動翻訳

Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.

タグ

Chondrogenic DifferentiationAdipose-derived Stem CellsCentrifugal GravityCartilage RepairPrechondrogenic DifferentiationOsteogenic DifferentiationFibrogenic DifferentiationCytokinesEDTAHemocytometerMicromass CultureChondrogenic Differentiation MediumTGF-beta-1

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

Immunomagnetic Separation of Fat Depot-specific Sca1high Adipose-derived Stem Cells (ASCs)

Induction of Acute Skeletal Muscle Regeneration by Cardiotoxin Injection

In Vitro Differentiation of Human Pluripotent Stem Cells into Trophoblastic Cells

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Derivation and Differentiation of Canine Ovarian Mesenchymal Stem Cells

Read Article