Mechanical stress can induce the chondrogenic differentiation of stem cells, providing a potential therapeutic approach for the repair of impaired cartilage. We present a protocol to induce the chondrogenic differentiation of adipose-derived stem cells (ASCs) using centrifugal gravity (CG). CG-induced upregulation of SOX9 results in the development of chondrogenic phenotypes.
Jang, Y., Jung, H., Ju, J. H. Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity. J. Vis. Exp. (120), e54934, doi:10.3791/54934 (2017).