ジャーナル
/
神経科学
/
Enumeration of Neural Stem Cells Using Clonal Assays
JoVE Journal
神経科学
This content is Free Access.
JoVE Journal 神経科学
Enumeration of Neural Stem Cells Using Clonal Assays

Enumeration of Neural Stem Cells Using Clonal Assays

DOI:
10.3791/54456-v

•

10:32 min

•

October 04, 2016

•

, , , , , , ,
1Neural Stem Cell Laboratory, Institute of Medical Biology,Agency for Science, Technology and Research (A*STAR)

章

  • 00:05標題
  • 00:50Culture of Clonal Sample Neurospheres
  • 02:07Preparation of Neurosphere-conditioned Medium
  • 04:20Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
  • 06:25Differentiation of Sample Neurospheres
  • 07:18Fixing the Differentiated Neurospheres
  • 08:16Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
  • 09:28Conclusion

概要

自動翻訳

自動翻訳

Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.

タグ

Neural Stem CellsClonal AssaysNeurosphere FormationNeural Stem Cell MarkersNeural ProgenitorsE14.5 MouseNSC Growth MediumTrypan-blue StainingHemocytometerPoly-L-lysineLamininNSC Differentiation Medium

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Growth and Differentiation of Adult Hippocampal Arctic Ground Squirrel Neural Stem Cells

Isolation and Expansion of the Adult Mouse Neural Stem Cells Using the Neurosphere Assay

Neural-Colony Forming Cell Assay: An Assay To Discriminate Bona Fide Neural Stem Cells from Neural Progenitor Cells

Generation of Neural Stem Cells from Discarded Human Fetal Cortical Tissue

Isolation and Culture of Neural Crest Stem Cells from Human Hair Follicles

The Neuroblast Assay: An Assay for the Generation and Enrichment of Neuronal Progenitor Cells from Differentiating Neural Stem Cell Progeny Using Flow Cytometry

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Feeder-free Derivation of Neural Crest Progenitor Cells from Human Pluripotent Stem Cells

Development of a Refined Protocol for Trans-scleral Subretinal Transplantation of Human Retinal Pigment Epithelial Cells into Rat Eyes

Isolation and Culture of Embryonic Mouse Neural Stem Cells

Read Article