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Enumeration of Neural Stem Cells Using Clonal Assays
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Enumeration of Neural Stem Cells Using Clonal Assays

Enumeration of Neural Stem Cells Using Clonal Assays

7,802 Views

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10:32 min

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October 04, 2016

DOI:

10.3791/54456-v

DOI:
10.3791/54456-v

•

10:32 min
October 04, 2016

•

3 Views
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1Neural Stem Cell Laboratory, Institute of Medical Biology,Agency for Science, Technology and Research (A*STAR)

Summary

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Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.

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Cite this Article

Narayanan, G., Yu, Y. H., Tham, M., Gan, H. T., Ramasamy, S., Sankaran, S., Hariharan, S., Ahmed, S. Enumeration of Neural Stem Cells Using Clonal Assays. J. Vis. Exp. (116), e54456, doi:10.3791/54456 (2016).

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