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Enumeration of Neural Stem Cells Using Clonal Assays
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Enumeration of Neural Stem Cells Using Clonal Assays
DOI:

10:32 min

October 04, 2016

, , , , , , ,

Chapitres

  • 00:05Titre
  • 00:50Culture of Clonal Sample Neurospheres
  • 02:07Preparation of Neurosphere-conditioned Medium
  • 04:20Transfer of Sample Neurospheres to the 50-well-chambered Coverslip
  • 06:25Differentiation of Sample Neurospheres
  • 07:18Fixing the Differentiated Neurospheres
  • 08:16Results: Differentiation of Clonal Neurospheres and Enumeration of NSC Frequency
  • 09:28Conclusion

Summary

Traduction automatique

Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.

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