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성인 쥐에서 본래 등쪽 뿌리 신경절에 패치 클램프 녹음
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生化学
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JoVE Journal 生化学
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

성인 쥐에서 본래 등쪽 뿌리 신경절에 패치 클램프 녹음

DOI:

12:00 min

September 29, 2016

, ,

  • 00:05標題
  • 00:55Dorsal Root Ganglia Dissection
  • 04:09Dorsal Root Ganglia Digestion
  • 06:11Patch Clamp Recordings
  • 10:05Results: Measurement of Neuronal Excitability and Ligand-induced Currents in Small DRG Neurons
  • 10:59Conclusion

概要

自動翻訳

This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

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