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Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
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Biochemie
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JoVE Journal Biochemie
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

13,832 Views

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12:00 min

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September 29, 2016

DOI:

10.3791/54287-v

DOI:
10.3791/54287-v

•

12:00 min
September 29, 2016

•

25 Views
, ,
1Department of Oral and Maxillofacial Surgery,University of California, San Francisco, 2Department of Anatomy,University of California, San Francisco

Summary

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This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

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Gong, K., Ohara, P. T., Jasmin, L. Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats. J. Vis. Exp. (115), e54287, doi:10.3791/54287 (2016).

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