ジャーナル
/
生化学
/
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats
JoVE Journal
生化学
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 生化学
Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats

13,839 Views

•

12:00 min

•

September 29, 2016

DOI:

10.3791/54287-v

DOI:
10.3791/54287-v

•

12:00 min
September 29, 2016

•

25 Views
, ,
1Department of Oral and Maxillofacial Surgery,University of California, San Francisco, 2Department of Anatomy,University of California, San Francisco

概要

Automatically generated

This manuscript describes how to prepare intact dorsal root ganglia for patch clamp recordings. This preparation maintains the microenvironment for neurons and satellite glial cells, thus avoiding the phenotypic and functional changes seen using dissociated DRG neurons.

Explore More Videos

Patch ClampDorsal Root GangliaAdult RatsPrimary Sensory NeuronsPainIntact PreparationSpinal NerveDorsal RootSatellite Glial CellsIn VivoAnesthesiaVertebral ColumnACSFSpinal CordL4L5EpineuriumRecording ChamberOxygenated ACSFPeristaltic Pump

Read Article

Cite this Article

Gong, K., Ohara, P. T., Jasmin, L. Patch Clamp Recordings on Intact Dorsal Root Ganglia from Adult Rats. J. Vis. Exp. (115), e54287, doi:10.3791/54287 (2016).

Download .ris file

Copy

Share Video

.

Copy