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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
JoVE Journal
生物学
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JoVE Journal 生物学
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

DOI:
10.3791/53508-v

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11:17 min

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January 07, 2016

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, , ,
1Department of Nanoscience,University of North Carolina at Greensboro, 2Center for Biotechnology, Genomics, and Health Research,University of North Carolina at Greensboro, 3HORIBA Scientific

章

  • 00:05標題
  • 01:15Prepare SPRi Chip for Antibody Array
  • 04:08SPRi Detection of Human Growth Hormone
  • 05:46ELISA Protocol
  • 08:21Results: SPRi, Nano-SPRi, and ELISA Analysis of Recombinant Human Growth Hormone
  • 09:53Conclusion

概要

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自動翻訳

The proposed work assesses the diagnostic potential of direct and amplified surface plasmon resonance imaging (SPRi) assays, particularly for the detection of recombinant human growth hormone in spiked human serum, by comparing SPRi results directly with commercially available enzyme-linked immunosorbent assay (ELISA) kit.

タグ

SPRiNano-SPRiELISAHGHSerumBiomarker DetectionLabel-freeReal-time MonitoringBiochip Surface Chemistry11-mercaptoundecanoic Acid1-Ethyl-3-carbodiimideN-Hydroxysuccinimide

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