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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
JoVE 杂志
生物学
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JoVE 杂志 生物学
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

16,074 Views

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11:17 min

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January 07, 2016

DOI:

10.3791/53508-v

DOI:
10.3791/53508-v

•

11:17 min
January 07, 2016

•

8 Views
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1Department of Nanoscience,University of North Carolina at Greensboro, 2Center for Biotechnology, Genomics, and Health Research,University of North Carolina at Greensboro, 3HORIBA Scientific

Summary

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The proposed work assesses the diagnostic potential of direct and amplified surface plasmon resonance imaging (SPRi) assays, particularly for the detection of recombinant human growth hormone in spiked human serum, by comparing SPRi results directly with commercially available enzyme-linked immunosorbent assay (ELISA) kit.

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Cite this Article

Vance, S., Zeidan, E., Henrich, V. C., Sandros, M. G. Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays. J. Vis. Exp. (107), e53508, doi:10.3791/53508 (2016).

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