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Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays
Journal JoVE
Biologie
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Journal JoVE Biologie
Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

Comparative Analysis of Human Growth Hormone in Serum Using SPRi, Nano-SPRi and ELISA Assays

DOI:
10.3791/53508-v

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11:17 min

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January 07, 2016

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, , ,
1Department of Nanoscience,University of North Carolina at Greensboro, 2Center for Biotechnology, Genomics, and Health Research,University of North Carolina at Greensboro, 3HORIBA Scientific

Chapitres

  • 00:05Titre
  • 01:15Prepare SPRi Chip for Antibody Array
  • 04:08SPRi Detection of Human Growth Hormone
  • 05:46ELISA Protocol
  • 08:21Results: SPRi, Nano-SPRi, and ELISA Analysis of Recombinant Human Growth Hormone
  • 09:53Conclusion

Summary

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The proposed work assesses the diagnostic potential of direct and amplified surface plasmon resonance imaging (SPRi) assays, particularly for the detection of recombinant human growth hormone in spiked human serum, by comparing SPRi results directly with commercially available enzyme-linked immunosorbent assay (ELISA) kit.

Tags

Keywords: SPRiNano-SPRiELISAHGHSerumBiomarker DetectionLabel-freeReal-time MonitoringBiochip Surface Chemistry11-mercaptoundecanoic Acid1-Ethyl-3-carbodiimideN-Hydroxysuccinimide

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