ジャーナル
/
免疫学と感染
/
Imaginologia todo animal e técnicas de citometria de fluxo para CD8 + respostas de células T Análise do antigénio específico da Nanoparticle após vacinação
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Imaginologia todo animal e técnicas de citometria de fluxo para CD8 + respostas de células T Análise do antigénio específico da Nanoparticle após vacinação

13,043 Views

•

11:07 min

•

April 29, 2015

DOI:

10.3791/52771-v

DOI:
10.3791/52771-v

•

11:07 min
April 29, 2015

•

1 Views
,
1Department of Pharmaceutical Sciences,University of Michigan, 2Biointerfaces Institute,University of Michigan, 3Department of Biomedical Engineering,University of Michigan

概要

Automatically generated

We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.

Explore More Videos

Nanoparticle VaccineCD8+ T CellWhole-animal ImagingFlow CytometryAntigen-specific T Cell ResponseInterbilayer-crosslinked Multilamellar Vesicles (ICMVs)Bioluminescence ImagingTetramer Staining

Read Article

Cite this Article

Ochyl, L. J., Moon, J. J. Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination. J. Vis. Exp. (98), e52771, doi:10.3791/52771 (2015).

Download .ris file

Copy

Share Video

.

Copy