Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Lukasz J. Ochyl; James J Moon

doi:10.3791/52771

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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

13,043 Views

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11:07 min

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April 29, 2015

DOI:

10.3791/52771-v

DOI:

10.3791/52771-v

•

11:07 min

April 29, 2015

•

1 Views

Lukasz J. Ochyl^1,2, James J Moon^1,2,3

¹Department of Pharmaceutical Sciences,University of Michigan, ²Biointerfaces Institute,University of Michigan, ³Department of Biomedical Engineering,University of Michigan

概要

We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.

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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

概要

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