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Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
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JoVE 杂志 免疫与感染
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

13,043 Views

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11:07 min

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April 29, 2015

DOI:

10.3791/52771-v

DOI:
10.3791/52771-v

•

11:07 min
April 29, 2015

•

1 Views
,
1Department of Pharmaceutical Sciences,University of Michigan, 2Biointerfaces Institute,University of Michigan, 3Department of Biomedical Engineering,University of Michigan

Summary

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We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.

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Nanoparticle VaccineCD8+ T CellWhole-animal ImagingFlow CytometryAntigen-specific T Cell ResponseInterbilayer-crosslinked Multilamellar Vesicles (ICMVs)Bioluminescence ImagingTetramer Staining

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Ochyl, L. J., Moon, J. J. Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination. J. Vis. Exp. (98), e52771, doi:10.3791/52771 (2015).

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