Journal
/
Inmunología y contagio
/
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

13,043 Views

•

11:07 min

•

April 29, 2015

DOI:

10.3791/52771-v

DOI:
10.3791/52771-v

•

11:07 min
April 29, 2015

•

1 Views
,
1Department of Pharmaceutical Sciences,University of Michigan, 2Biointerfaces Institute,University of Michigan, 3Department of Biomedical Engineering,University of Michigan

Summary

Automatically generated

We describe whole-animal imaging and flow cytometry-based techniques for monitoring expansion of antigen-specific CD8+ T cells in response to immunization with nanoparticles in a murine model of vaccination.

Explore More Videos

Nanoparticle VaccineCD8+ T CellWhole-animal ImagingFlow CytometryAntigen-specific T Cell ResponseInterbilayer-crosslinked Multilamellar Vesicles (ICMVs)Bioluminescence ImagingTetramer Staining

Read Article

Cite this Article

Ochyl, L. J., Moon, J. J. Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination. J. Vis. Exp. (98), e52771, doi:10.3791/52771 (2015).

Download .ris file

Copy

Share Video

.

Copy