Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Olivier Etienne; Amandine Bery; Telma Roque; Chantal Desmaze; Fran&#231;ois D. Boussin

doi:10.3791/51209

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Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

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神経科学

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JoVE Journal 神経科学

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

15,117 Views

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09:51 min

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May 07, 2014

DOI:

10.3791/51209-v

DOI:

10.3791/51209-v

•

09:51 min

May 07, 2014

•

9 Views

Olivier Etienne^1,2,3,4, Amandine Bery^1,2,3,4, Telma Roque^1,2,3,4, Chantal Desmaze^1,2,3,4, François D. Boussin^1,2,3,4

¹Laboratoire de Radiopathologie,CEA DSV iRCM SCSR, ²INSERM, U967, ³Université Paris Diderot, Sorbonne Paris Cité, ⁴Université Paris Sud, UMR 967

概要

Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.

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Neural Stem Cells Neural Progenitor Cells Cell Cycle Genotoxic Stress Ionizing Radiation EdU BrdU DNA Damage Response Cell Cycle Checkpoint Dorsal Telencephalon

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Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

概要

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