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Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
章
- 00:05標題
- 01:13Brain Slice Preparation
- 03:03EdU and BrdU Staining
- 06:05Imaging and Analysis
- 06:59Identifying Apoptotic Cells and Cell Cycle Phase
- 09:10Conclusion
Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.
