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Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
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Neurosciences
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Journal JoVE Neurosciences
Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

DOI:
10.3791/51209-v

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09:51 min

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May 07, 2014

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, , , ,
1Laboratoire de Radiopathologie,CEA DSV iRCM SCSR, 2INSERM, U967, 3Université Paris Diderot, Sorbonne Paris Cité, 4Université Paris Sud, UMR 967

Chapitres

  • 00:05Titre
  • 01:13Brain Slice Preparation
  • 03:03EdU and BrdU Staining
  • 06:05Imaging and Analysis
  • 06:59Identifying Apoptotic Cells and Cell Cycle Phase
  • 09:10Conclusion

Summary

Traduction automatique

Traduction automatique

Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.

Tags

Keywords: Neural Stem CellsNeural Progenitor CellsCell CycleGenotoxic StressIonizing RadiationEdUBrdUDNA Damage ResponseCell Cycle CheckpointDorsal Telencephalon

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