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Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress
JoVE 杂志
神经科学
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JoVE 杂志 神经科学
Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress

15,117 Views

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09:51 min

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May 07, 2014

DOI:

10.3791/51209-v

DOI:
10.3791/51209-v

•

09:51 min
May 07, 2014

•

9 Views
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1Laboratoire de Radiopathologie,CEA DSV iRCM SCSR, 2INSERM, U967, 3Université Paris Diderot, Sorbonne Paris Cité, 4Université Paris Sud, UMR 967

Summary

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Administration of two analogs of thymidine, EdU and BrdU, in pregnant mice allows the analysis of cell cycle progression in neural and progenitor cells in the embryonic mouse brain. This method is useful to determine the effects of genotoxic stress, including ionizing radiation, during brain development.

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Cite this Article

Etienne, O., Bery, A., Roque, T., Desmaze, C., Boussin, F. D. Assessing Cell Cycle Progression of Neural Stem and Progenitor Cells in the Mouse Developing Brain after Genotoxic Stress. J. Vis. Exp. (87), e51209, doi:10.3791/51209 (2014).

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