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Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter
JoVE Journal
生物学
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JoVE Journal 生物学
Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

DOI:
10.3791/51178-v

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07:37 min

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February 13, 2014

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, , ,
1Microbial Biotechnology, Institute Biology Leiden,Leiden University, 2Microbiology, Kluyver Centre for Genomics of Industrial Fermentation,Utrecht University

章

  • 00:05標題
  • 00:59COPAS Analysis
  • 03:35Pellet Sorting
  • 05:06Results: Measurement and Sorting of Streptomyces Pellets by COPAS
  • 07:13Conclusion

概要

自動翻訳

自動翻訳

Liquid-grown Streptomyces cultures are characterized by mycelial pellets that are heterogeneous in size. We here describe a method to analyze and sort such pellets in a high-throughput manner. These pellets can be used for further analyses, which will provide leads to understand and control growth heterogeneity.

タグ

StreptomycesCell PelletsComplex Object Parametric Analyzer And Sorter (COPAS)Mycelial PelletsPellet SizeCell FactoryBioreactorFilamentous BacteriaEnzyme ProductionAntibiotic ProductionHeterogeneous GrowthProductivity

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