Liquid-grown Streptomyces cultures are characterized by mycelial pellets that are heterogeneous in size. We here describe a method to analyze and sort such pellets in a high-throughput manner. These pellets can be used for further analyses, which will provide leads to understand and control growth heterogeneity.
Petrus, M. L. C., van Veluw, G. J., Wösten, H. A. B., Claessen, D. Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter. J. Vis. Exp. (84), e51178, doi:10.3791/51178 (2014).