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Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter
Journal JoVE
Biologie
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Journal JoVE Biologie
Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

Sorting of Streptomyces Cell Pellets Using a Complex Object Parametric Analyzer and Sorter

DOI:
10.3791/51178-v

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07:37 min

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February 13, 2014

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, , ,
1Microbial Biotechnology, Institute Biology Leiden,Leiden University, 2Microbiology, Kluyver Centre for Genomics of Industrial Fermentation,Utrecht University

Chapitres

  • 00:05Titre
  • 00:59COPAS Analysis
  • 03:35Pellet Sorting
  • 05:06Results: Measurement and Sorting of Streptomyces Pellets by COPAS
  • 07:13Conclusion

Summary

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Traduction automatique

Liquid-grown Streptomyces cultures are characterized by mycelial pellets that are heterogeneous in size. We here describe a method to analyze and sort such pellets in a high-throughput manner. These pellets can be used for further analyses, which will provide leads to understand and control growth heterogeneity.

Tags

StreptomycesCell PelletsComplex Object Parametric Analyzer And Sorter (COPAS)Mycelial PelletsPellet SizeCell FactoryBioreactorFilamentous BacteriaEnzyme ProductionAntibiotic ProductionHeterogeneous GrowthProductivity

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