Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Helmar Waiczies; Martin Guenther; Julia Skodowski; Stefano Lepore; Andreas Pohlmann; Thoralf Niendorf; Sonia Waiczies

doi:10.3791/50251

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免疫学と感染

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Monitoraggio migrazione delle cellule dendritiche usando 19 F / 1 H Magnetic Resonance Imaging

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JoVE Journal 免疫学と感染

Monitoring Dendritic Cell Migration using ¹⁹F / ¹H Magnetic Resonance Imaging

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Monitoraggio migrazione delle cellule dendritiche usando 19 F / 1 H Magnetic Resonance Imaging

11,692 Views

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08:12 min

•

March 20, 2013

DOI:

10.3791/50251-v

DOI:

10.3791/50251-v

•

08:12 min

March 20, 2013

•

4 Views

Helmar Waiczies^1,2, Martin Guenther^1,2, Julia Skodowski^1,2, Stefano Lepore^1,2, Andreas Pohlmann², Thoralf Niendorf^1,2, Sonia Waiczies^1,2

¹Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, ²Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

概要

Monitoraggio delle cellule utilizzando la risonanza magnetica ha guadagnato notevole attenzione negli ultimi anni. Questo protocollo descrive l'etichettatura delle cellule dendritiche con fluoro ( 19 Particelle F)-ricchi, l'applicazione in vivo di queste cellule, e monitorare l'entità della loro migrazione verso il linfonodo drenante con 19 F / 1 H MRI e 19 F MRS.

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Monitoraggio migrazione delle cellule dendritiche usando<sup> 19</sup> F /<sup> 1</sup> H Magnetic Resonance Imaging

概要

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