Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Saijilafu; Feng-Quan Zhou

doi:10.3791/4141

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神経科学

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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

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神経科学

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JoVE Journal 神経科学

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

26,322 Views

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09:42 min

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August 17, 2012

DOI:

10.3791/4141-v

DOI:

10.3791/4141-v

•

09:42 min

August 17, 2012

•

7 Views

Saijilafu¹, Feng-Quan Zhou^1,2

¹Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, ²Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

概要

An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

概要

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