Journal
/
神经科学
/
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
JoVE 杂志
神经科学
需要订阅 JoVE 才能查看此.  登录或开始免费试用。
JoVE 杂志 神经科学
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

26,330 Views

•

09:42 min

•

August 17, 2012

DOI:

10.3791/4141-v

DOI:
10.3791/4141-v

•

09:42 min
August 17, 2012

•

7 Views
,
1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Summary

Automatically generated

An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

Explore More Videos

Genetic StudyAxon RegenerationCultured Adult Dorsal Root Ganglion NeuronsCentral Nervous System (CNS)Peripheral Nervous System (PNS)Mature NeuronsAxon GrowthHostile EnvironmentPeripheral Nerve InjuriesConditioning LesionSpinal Cord InjuryMolecular MechanismsCNS Axon Regeneration

Read Article

Cite this Article

Saijilafu, Zhou, F. Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons. J. Vis. Exp. (66), e4141, doi:10.3791/4141 (2012).

Download .ris file

Copy

Share Video

.

Copy