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Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons
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Neurociencias
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JoVE Revista Neurociencias
Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons

26,315 Views

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09:42 min

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August 17, 2012

DOI:

10.3791/4141-v

DOI:
10.3791/4141-v

•

09:42 min
August 17, 2012

•

7 Views
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1Department of Orthopaedic Surgery,Johns Hopkins University School of Medicine, 2Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine

Summary

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An in vitro model for genetic study of axon regeneration using cultured adult mouse dorsal root ganglion neurons is described. The method includes a re-suspension/re-plating step to allow axon re-growth from neurons undergoing genetic manipulation. This approach is especially useful for loss-of-function studies of axon regeneration using RNAi-based protein knockdown.

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Genetic StudyAxon RegenerationCultured Adult Dorsal Root Ganglion NeuronsCentral Nervous System (CNS)Peripheral Nervous System (PNS)Mature NeuronsAxon GrowthHostile EnvironmentPeripheral Nerve InjuriesConditioning LesionSpinal Cord InjuryMolecular MechanismsCNS Axon Regeneration

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Cite this Article

Saijilafu, Zhou, F. Genetic Study of Axon Regeneration with Cultured Adult Dorsal Root Ganglion Neurons. J. Vis. Exp. (66), e4141, doi:10.3791/4141 (2012).

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