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Introducing Shear Stress in the Study of Bacterial Adhesion
JoVE Journal
免疫学と感染
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JoVE Journal 免疫学と感染
Introducing Shear Stress in the Study of Bacterial Adhesion

Introducing Shear Stress in the Study of Bacterial Adhesion

DOI:
10.3791/3241-v

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13:28 min

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September 02, 2011

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1Blood vessels as a target for infection, Paris center for cardiovascular research,INSERM U970

章

  • 00:05標題
  • 01:32Human Host Cell and Bacterial Culture
  • 03:21Initial Adhesion of Individual Bacteria to Host Cells
  • 06:41Quantification of Initial Adhesion of Individual Bacteria to Host Cells
  • 07:50Measuring the Resistance to Flow of Individual Bacteria Adherent to Host Cells
  • 08:23Measuring the Growth of an Isolated Adherent Bacterium to a Microcolony
  • 09:28Measuring the Resistance to Flow of Adherent Bacterial Microcolonies
  • 11:14Bacterial Detachment from Microcolonies
  • 11:57Bacterial Adhesion and Attachment: Representative Data
  • 12:49Conclusion

概要

自動翻訳

自動翻訳

During the infection process, a key step is the adhesion of pathogens with host cells. In most instances this adhesion step occurs in the presence of mechanical stress generated by flowing liquid. We describe a technique that introduces shear stress as an important parameter in the study of bacterial adhesion.

タグ

Shear StressBacterial AdhesionPathogenesisHost Cell SurfaceFlowing LiquidMucusBlood CirculationEndothelial CellsMechanical ForceShear Stress IntensityCapillaries

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