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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
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May 19, 2010
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We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.
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Jiang, P., Selvaraj, V., Deng, W. Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors. J. Vis. Exp. (39), e1960, doi:10.3791/1960 (2010).
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