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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
Journal JoVE
Biologie
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Journal JoVE Biologie
Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors

DOI:
10.3791/1960-v

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08:11 min

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May 19, 2010

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, ,
1Department of Cell Biology and Human Anatomy Institute for Pediatric Regenerative Medicine,School of Medicine, University of California, Davis

Chapitres

  • 00:00Titre
  • 01:03Introduction
  • 01:49Passaging Mouse Embryonic Stem Cells
  • 03:18Generation Of Oligodendrocyte Precursor Cells From Mouse ES Cells
  • 05:40Generation of Spiking Oligodendrocyte Precursor Cells
  • 06:47Representative Results
  • 07:31Conclusion

Summary

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We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.

Tags

Embryonic Stem CellsOligodendrocyte PrecursorsMyelinating CellsCentral Nervous SystemRegenerative Cell TherapyDemyelinating DiseasesTransplantationTranscription Factor Olig2Proteoglycan NG2GFP-Olig2 Mouse Embryonic Stem Cell Reporter LineDifferentiationSmall MoleculesRetinoic AcidSonic Hedgehog Agonist PurmorphamineElectrophysiological PropertyNaV1.2 Subunit

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