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Differentiation of Embryonic Stem Cells into Oligodendrocyte Precursors
Kapitel
- 00:00Titel
- 01:03Introduction
- 01:49Passaging Mouse Embryonic Stem Cells
- 03:18Generation Of Oligodendrocyte Precursor Cells From Mouse ES Cells
- 05:40Generation of Spiking Oligodendrocyte Precursor Cells
- 06:47Representative Results
- 07:31Conclusion
We describe a small molecule-based protocol for differentiation of mouse embryonic stem cells into oligodendrocyte precursor cells (OPCs). This protocol generates Olig2+NG2+ OPCs with high efficiency by 30 days of differentiation. We also describe a method to generate "spiking" OPCs that can fire action potentials.
Tags
Embryonic Stem CellsOligodendrocyte PrecursorsMyelinating CellsCentral Nervous SystemRegenerative Cell TherapyDemyelinating DiseasesTransplantationTranscription Factor Olig2Proteoglycan NG2GFP-Olig2 Mouse Embryonic Stem Cell Reporter LineDifferentiationSmall MoleculesRetinoic AcidSonic Hedgehog Agonist PurmorphamineElectrophysiological PropertyNaV1.2 Subunit
